Large-scale genetic perturbations reveal regulatory networks and an abundance of gene-specific repressors.
To understand regulatory systems, it would be useful to uniformly determine how different components contribute to the expression of all other genes. We therefore monitored mRNA expression genome-wide, for individual deletions of one-quarter of yeast genes, focusing on (putative) regulators. The resulting genetic perturbation signatures reflect many different properties. These include the architecture of protein […]
Systematic integrative genomics, transcriptomics and ultra-deep proteomics analysis reveals novel insight in disease biology
Experts in proteomics from Utrecht University and next-generation DNA sequencing from the Hubrecht Institute/UMC Utrecht collaborated in a study to systematically determine the consequences of genetic variation on the transcriptome and proteome. The researchers applied ultra-deep quantitative proteomics, whole genome sequencing and RNA-seq to liver tissues from different inbred rat strains. One of these inbred […]